Journal: Bioactive Materials

Article Title: Spatiotemporally engineered microneedle for microenvironment remodeling propels mucosal regeneration after tracheal mucosal injury

doi: 10.1016/j.bioactmat.2026.01.026

Figure Lengend Snippet: Evaluation of mucosal status after tracheal mucosal injury in rabbits . (a) Gross morphology and Masson staining of mucosal defects (n = 3). (b – f) Tracheal samples were collected on Day 10, and RNA sequencing was performed to assess biological differences between Native and Model groups (n = 3). (b) Principal components analysis of samples. (c) Volcano plot of DEGs. (d) KEGG enrichment analysis of DEGs. (e) Chord plot of enriched KEGG terms. (f) Heatmaps of DEGs associated with inflammation and oxidative stress. (g) IF staining of FISH (marker of bacteria, pink), iNOS (marker of M1 macrophages, orange), CD206 (marker of M2 macrophages, green), and immunohistochemical staining of CD31 (marker of endothelial cells) in various samples, blood vessels are denoted by triangles.

Article Snippet: Immunofluorescence staining of CK14 (Abcam, ab181595), AC-Tub (Proteintech, 66200-1-Ig), ZO-1 (Proteintech, 21773-1-AP), and Immunohistochemical (IHC) staining for CD31 (Servicebio, S1002) were conducted to reveal the conditions of mucosal regeneration, according to previous literature [ ].

Techniques: Staining, RNA Sequencing, Marker, Bacteria, Immunohistochemical staining

Journal: Bioactive Materials

Article Title: Spatiotemporally engineered microneedle for microenvironment remodeling propels mucosal regeneration after tracheal mucosal injury

doi: 10.1016/j.bioactmat.2026.01.026

Figure Lengend Snippet: Gel-AgNA/MgGA MN remodel the microenvironment after TMI by regulating infection, inflammation, oxidative stress, and vascular disruption. (a) FISH (marker of bacteria, red) staining of harvested samples after treated with Gel, Gel-AgNA, Gel-MgGA, and Gel-AgNA/MgGA MN. (b, c) Quantitative analysis of bacterial coverage and fluorescence intensity of FISH (n = 9). (d) Heatmaps of DEGs related to inflammation and oxidative stress. (e) RT-PCR analysis showing the relative expression levels of IL-1, TNF-α, HIF-1α, and IL-6 (n = 3). (f) IF staining of iNOS (marker of M1 macrophages, red) and CD206 (marker of M2 macrophages, green). (g) Quantitative analysis of iNOS and CD206 fluorescence intensity. (h) RT-PCR analysis showing the relative expression levels of Arg1 and CD206. (i) IF staining of CD31 (marker of blood vessels, red). (j, k) RT-PCR analysis showing the relative expression levels of VEGF and HIF-1α (n = 3).

Article Snippet: Immunofluorescence staining of CK14 (Abcam, ab181595), AC-Tub (Proteintech, 66200-1-Ig), ZO-1 (Proteintech, 21773-1-AP), and Immunohistochemical (IHC) staining for CD31 (Servicebio, S1002) were conducted to reveal the conditions of mucosal regeneration, according to previous literature [ ].

Techniques: Infection, Disruption, Marker, Bacteria, Staining, Fluorescence, Reverse Transcription Polymerase Chain Reaction, Expressing

Journal: Aging Cell

Article Title: Multi‐Omics Reveals Dysregulated Neurotransmitter Systems in Aging and CNS Disorders

doi: 10.1111/acel.70544

Figure Lengend Snippet: Age‐dependent NTS molecule expression patterns across cell types. (A) Relative expression of NTS across cell types (ExN, InN, MOL, OPC, Astro, Micro, Endo, Peri) as a function of age. NTS include acetylcholine, cannabinoid, dopamine, GABA, glutamate, glycine, histamine, MAT (monoamine transporters), norepinephrine, opioid, progestin, prostaglandin, and serotonin. Notable trends include age‐associated increases or decreases in expression levels of specific neurotransmitters within distinct cell types. (B) Immunohistochemical staining of HTR1E in cerebral cortex tissue (HPA004931). Images show representative staining in male (age 45) and female (age 54) samples. Scale bar: 100 μm. (C) Scatter plot of normalized TPM (nTPM) values showing HTR1E expression levels in frontal cortex samples (BA9, n = 209). Samples are stratified into three clusters: HTR1E high , HTR1E middle , and HTR1E low . (D) Bar plot showing the distribution of HTR1E expression clusters across young and old groups. The proportion of samples in each cluster is displayed. (E) Immunohistochemical staining of PGRMC1 in cerebral cortex tissue (HPA002877). Images show representative staining in female (age 45) and female (age 54) samples. Insets highlight specific regions with detailed staining patterns. Scale bar: 100 μm. (F) Scatter plot of normalized TPM (nTPM) values showing PGRMC1 expression levels in frontal cortex samples (BA9, n = 209). Samples are stratified into three clusters: PGRMC1 high , PGRMC1 middle , and PGRMC1 low . (G) Bar plot showing the distribution of PGRMC1 expression clusters across young and old groups. The proportion of samples in each cluster is displayed.

Article Snippet: Immunohistochemical staining images and frontal cortex RNA‐seq data from the Human Protein Atlas (HPA) provided supportive cross‐dataset validation for the snRNA‐seq findings.

Techniques: Expressing, Immunohistochemical staining, Staining